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Robust induction of primordial germ cells of white rhinoceros on the brink of extinction

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Item Type:Article
Title:Robust induction of primordial germ cells of white rhinoceros on the brink of extinction
Creators Name:Hayashi, M. and Zywitza, V. and Naitou, Y. and Hamazaki, N. and Goeritz, F. and Hermes, R. and Holtze, S. and Lazzari, G. and Galli, C. and Stejskal, J. and Diecke, S. and Hildebrandt, T.B. and Hayashi, K.
Abstract:In vitro gametogenesis, the process of generating gametes from pluripotent cells in culture, is a powerful tool for improving our understanding of germ cell development and an alternative source of gametes. Here, we induced primordial germ cell-like cells (PGCLCs) from pluripotent stem cells of the northern white rhinoceros (NWR), a species for which only two females remain, and southern white rhinoceros (SWR), the closest species to the NWR. PGCLC differentiation from SWR embryonic stem cells is highly reliant on bone morphogenetic protein and WNT signals. Genetic analysis revealed that SRY-box transcription factor 17 (SOX17) is essential for SWR-PGCLC induction. Under the defined condition, NWR induced pluripotent stem cells differentiated into PGCLCs. We also identified cell surface markers, CD9 and Integrin subunit alpha 6 (ITGA6), that enabled us to isolate PGCLCs without genetic alteration in pluripotent stem cells. This study provides a first step toward the production of NWR gametes in culture and understanding of the basic mechanism of primordial germ cell specification in a large animal.
Keywords:Cell Differentiation, Embryonic Stem Cells, Germ Cells, Induced Pluripotent Stem Cells, Pluripotent Stem Cells, Animals
Source:Science Advances
Publisher:American Association for the Advancement of Science
Page Range:eabp9683
Date:9 December 2022
Official Publication:https://doi.org/10.1126/sciadv.abp9683
PubMed:View item in PubMed

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