The lncRNA landscape of cardiac resident macrophages and identification of Schlafenlnc as a regulator of macrophage migratory function

Item Type:	Preprint
Title:	The lncRNA landscape of cardiac resident macrophages and identification of Schlafenlnc as a regulator of macrophage migratory function
Creators Name:	Dueck, A. and Althaus, L. and Heise, K. and Esfandyari, D. and Bayguen, S. and Brandes, R.P. and Gagneur, J. and Jae, N. and Knolle, P. and Leisegang, M.S. and Maegdefessel, L. and Meitinger, T. and Petzold, N. and Ramanujam, D. and Sager, H. and Schulz, C. and Theodorakis, E. and Uzonyi, A. and Weinberger, T. and Bader, M. and Schmidt-Supprian, M. and Engelhardt, S.
Abstract:	Cardiac resident macrophages (crMPs) were recently shown to exert pivotal functions in cardiac homeostasis and disease, but the underlying molecular mechanisms are largely unclear. Long non-coding RNAs (lncRNAs) are increasingly recognized as important regulatory molecules in a number of cell types, but neither the identity nor the molecular mechanisms of lncRNAs in crMPs are known. Here, we have employed deep RNA-seq and single cell RNA sequencing to resolve the crMP lncRNA landscape from healthy and diseased murine myocardium. CrMPs express previously unknown and highly cell type-specific lncRNAs, among which one lncRNA, termed Schlafenlnc, was particularly abundant and enriched in crMPs. We found Schlafenlnc to be necessary for migration-associated gene expression in macrophages in vitro and in vivo and essential for their adhesion and migration. Collectively, our data provide a basis to the systematic characterization of lncRNAs in crMPs and establish Schlafenlnc as a critical regulator of macrophage migratory functions.
Keywords:	Long Non-coding RNA, Cardiac Resident Macrophages, Pressure Overload, SchlafenInc, Single Cell Sequencing, Animals, Mice
Source:	bioRxiv
Publisher:	Cold Spring Harbor Laboratory Press
Article Number:	2022.11.30.518576
Date:	22 December 2022
Official Publication:	https://doi.org/10.1101/2022.11.30.518576

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