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| Item Type: | Article |
|---|---|
| Title: | Generation of induced pluripotent stem cells from three individuals with Huntington's disease |
| Creators Name: | Miller, D.C. and Lisowski, P. and Lickfett, S. and Mlody, B. and Bünning, M. and Genehr, C. and Ulrich, C. and Wanker, E.E. and Diecke, S. and Priller, J. and Prigione, A. |
| Abstract: | Huntington's disease (HD) is a neurodegenerative disorder caused by abnormal glutamine (Q) expansion in the huntingtin protein due to elongated CAG repeats in the gene HTT. We used non-integrative episomal plasmids to generate induced pluripotent stem cells (iPSCs) from three individuals affected by HD: CH1 (58Q), and two twin brothers CH3 (44Q) and CH4 (44Q). The iPSC lines exhibited one healthy HTT allele and one with elongated CAG repeats, as confirmed by PCR and sequencing. All iPSC lines expressed pluripotency markers, exhibited a normal karyotype, and generated cells of the three germ layers in vitro. |
| Keywords: | Huntington Disease, Induced Pluripotent Stem Cells, Siblings |
| Source: | Stem Cell Research |
| ISSN: | 1873-5061 |
| Publisher: | Elsevier |
| Volume: | 65 |
| Page Range: | 102976 |
| Date: | December 2022 |
| Official Publication: | https://doi.org/10.1016/j.scr.2022.102976 |
| PubMed: | View item in PubMed |
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