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Generation of induced pluripotent stem cells from three individuals with Huntington's disease

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Item Type:Article
Title:Generation of induced pluripotent stem cells from three individuals with Huntington's disease
Creators Name:Miller, D.C., Lisowski, P., Lickfett, S., Mlody, B., Bünning, M., Genehr, C., Ulrich, C., Wanker, E.E., Diecke, S., Priller, J. and Prigione, A.
Abstract:Huntington's disease (HD) is a neurodegenerative disorder caused by abnormal glutamine (Q) expansion in the huntingtin protein due to elongated CAG repeats in the gene HTT. We used non-integrative episomal plasmids to generate induced pluripotent stem cells (iPSCs) from three individuals affected by HD: CH1 (58Q), and two twin brothers CH3 (44Q) and CH4 (44Q). The iPSC lines exhibited one healthy HTT allele and one with elongated CAG repeats, as confirmed by PCR and sequencing. All iPSC lines expressed pluripotency markers, exhibited a normal karyotype, and generated cells of the three germ layers in vitro.
Keywords:Huntington Disease, Induced Pluripotent Stem Cells, Siblings
Source:Stem Cell Research
ISSN:1873-5061
Publisher:Elsevier
Volume:65
Page Range:102976
Date:December 2022
Official Publication:https://doi.org/10.1016/j.scr.2022.102976
PubMed:View item in PubMed

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