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| Item Type: | Article |
|---|---|
| Title: | Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy |
| Creators Name: | Trumpp, M. and Oliveras, A. and Gonschior, H. and Ast, J. and Hodson, D.J. and Knaus, P. and Lehmann, M. and Birol, M. and Broichhagen, J. |
| Abstract: | Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy. |
| Keywords: | Fluorescence Resonance Energy Transfer, Microscopy, Nanotechnology, Proteins |
| Source: | Chemical Communications |
| ISSN: | 1359-7345 |
| Publisher: | Royal Society of Chemistry |
| Volume: | 58 |
| Number: | 99 |
| Page Range: | 13724-13727 |
| Date: | 13 December 2022 |
| Official Publication: | https://doi.org/10.1039/d2cc04823j |
| PubMed: | View item in PubMed |
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