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| Item Type: | Article |
|---|---|
| Title: | Negative selection, not receptor editing, is a physiological response of autoreactive thymocytes |
| Creators Name: | Kreslavsky, T. and Kim, H.J. and Koralov, S.B. and Ghitza, D. and Buch, T. and Cantor, H. and Rajewsky, K. and von Boehmer, H. |
| Abstract: | Antigen receptor editing—a process of secondary rearrangements of antigen receptor genes in autoreactive lymphocytes—is a well-established tolerance mechanism in B cells, whereas its role in T cells remains controversial. Here, we investigated this issue using a novel Tcra knock-in locus, which ensured appropriate timing of TCRα expression and allowed secondary rearrangements. Under these conditions the only response to self-antigen that could be unambiguously identified was negative selection of CD4/CD8 double positive thymocytes. No evidence could be obtained for antigen-induced TCR editing, whereas replacement of the transgenic TCRα chain by ongoing gene rearrangement occurred in some cells irrespective of the presence or absence of self-antigen. |
| Keywords: | alpha-beta T-Cell Antigen Receptors, alpha-Chain T-Cell Antigen Receptor Gene Rearrangement, Antigen-Mediated Clonal Selection, Autoantigens, Gene Order, Gene Targeting, Genetic Loci, Phenotype, Transgenic Mice, Thymocytes, Animals, Mice |
| Source: | Journal of Experimental Medicine |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Volume: | 210 |
| Number: | 10 |
| Page Range: | 1911-1918 |
| Date: | 23 September 2013 |
| Official Publication: | https://doi.org/10.1084/jem.20130876 |
| PubMed: | View item in PubMed |
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