Braveheart, a long noncoding RNA required for cardiovascular lineage commitment

Item Type:	Article
Title:	Braveheart, a long noncoding RNA required for cardiovascular lineage commitment
Creators Name:	Klattenhoff, C.A. and Scheuermann, J.C. and Surface, L.E. and Bradley, R.K. and Fields, P.A. and Steinhauser, M.L. and Ding, H. and Butty, V.L. and Torrey, L. and Haas, S. and Abo, R. and Tabebordbar, M. and Lee, R.T. and Burge, C.B. and Boyer, L.A.
Abstract:	Long noncoding RNAs (lncRNAs) are often expressed in a development-specific manner, yet little is known about their roles in lineage commitment. Here, we identified Braveheart (Bvht), a heart-associated lncRNA in mouse. Using multiple embryonic stem cell (ESC) differentiation strategies, we show that Bvht is required for progression of nascent mesoderm toward a cardiac fate. We find that Bvht is necessary for activation of a core cardiovascular gene network and functions upstream of mesoderm posterior 1 (MesP1), a master regulator of a common multipotent cardiovascular progenitor. We also show that Bvht interacts with SUZ12, a component of polycomb-repressive complex 2 (PRC2), during cardiomyocyte differentiation, suggesting that Bvht mediates epigenetic regulation of cardiac commitment. Finally, we demonstrate a role for Bvht in maintaining cardiac fate in neonatal cardiomyocytes. Together, our work provides evidence for a long noncoding RNA with critical roles in the establishment of the cardiovascular lineage during mammalian development.
Keywords:	Basic Helix-Loop-Helix Transcription Factors, Cell Differentiation, Embryonic Stem Cells, Gene Regulatory Networks, Mesoderm, Cardiac Myocytes, Polycomb Repressive Complex 2, Long Noncoding RNA, Animals, Mice, Rats
Source:	Cell
ISSN:	0092-8674
Publisher:	Cell Press
Volume:	152
Number:	3
Page Range:	570-83
Date:	31 January 2013
Official Publication:	https://doi.org/10.1016/j.cell.2013.01.003
PubMed:	View item in PubMed

Repository Staff Only: item control page