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Pan-claudin family interactome analysis reveals shared and specific interactions

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Item Type:Article
Title:Pan-claudin family interactome analysis reveals shared and specific interactions
Creators Name:Suarez Artiles, L., Breiderhoff, T., Girardello, R., Gonschior, H., Rodius, S., Lesur, A., Reimer, U., Ramberger, E., Perez-Hernandez, D., Müller, D., Mertins, P. and Dittmar, G.
Abstract:Claudins are a family of transmembrane proteins expressed in epithelial tissues and are the major components of tight junctions (TJs), which define barrier properties in epithelia and maintain cell polarity. How claudins regulate the formation of TJs and which functions they exert outside of them is not entirely understood. Although the long and unstructured C-terminal tail is essential for regulation, it is unclear how it is involved in these functions beyond interacting with TJ-associated proteins such as TJ protein ZO-1 (TJP1). Here, we present an interactome study of the pan-claudin family in Madin-Darby canine kidney (MDCK)-C7 cells by combining two complementary mass spectrometry-based pull-down techniques creating an interaction landscape of the entire claudin family. The interaction partners of the claudins' C termini reveal their possible implications in localized biological processes in epithelial cells and their regulation by post-translational modifications (PTMs).
Keywords:Claudin, Interactome, Claudinome, Prisma, Mass Spectrometry, Tight Junction, Proteasome, TRiC, Claudin Family, Animals, Dogs
Source:Cell Reports
ISSN:2211-1247
Publisher:Cell Press / Elsevier
Volume:41
Number:6
Page Range:111588
Date:8 November 2022
Official Publication:https://doi.org/10.1016/j.celrep.2022.111588
PubMed:View item in PubMed

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