Chemically defined generation of human cardiomyocytes

Item Type:	Article
Title:	Chemically defined generation of human cardiomyocytes
Creators Name:	Burridge, P.W. and Matsa, E. and Shukla, P. and Lin, Z.C. and Churko, J.M. and Ebert, A.D. and Lan, F. and Diecke, S. and Huber, B. and Mordwinkin, N.M. and Plews, J.R. and Abilez, O.J. and Cui, B. and Gold, J.D. and Wu, J.C.
Abstract:	Existing methods for human induced pluripotent stem cell (hiPSC) cardiac differentiation are efficient but require complex, undefined medium constituents that hinder further elucidation of the molecular mechanisms of cardiomyogenesis. Using hiPSCs derived under chemically defined conditions on synthetic matrices, we systematically developed an optimized cardiac differentiation strategy, using a chemically defined medium consisting of just three components: the basal medium RPMI 1640, L-ascorbic acid 2-phosphate and rice-derived recombinant human albumin. Along with small molecule-based induction of differentiation, this protocol produced contractile sheets of up to 95% TNNT2(+) cardiomyocytes at a yield of up to 100 cardiomyocytes for every input pluripotent cell and was effective in 11 hiPSC lines tested. This chemically defined platform for cardiac specification of hiPSCs will allow the elucidation of cardiomyocyte macromolecular and metabolic requirements and will provide a minimal system for the study of maturation and subtype specification.
Keywords:	Stem-Cell Differentiation, Stem-Cell Research
Source:	Nature Methods
ISSN:	1548-7091
Publisher:	Nature Publishing Group
Volume:	11
Number:	8
Page Range:	855-60
Date:	August 2014
Official Publication:	https://doi.org/10.1038/nmeth.2999
PubMed:	View item in PubMed

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