Second generation codon optimized minicircle (CoMiC) for nonviral reprogramming of human adult fibroblasts

Item Type:	Article
Title:	Second generation codon optimized minicircle (CoMiC) for nonviral reprogramming of human adult fibroblasts
Creators Name:	Diecke, S. and Lisowski, L. and Kooreman, N.G. and Wu, J.C.
Abstract:	The ability to induce pluripotency in somatic cells is one of the most important scientific achievements in the fields of stem cell research and regenerative medicine. This technique allows researchers to obtain pluripotent stem cells without the controversial use of embryos, providing a novel and powerful tool for disease modeling and drug screening approaches. However, using viruses for the delivery of reprogramming genes and transcription factors may result in integration into the host genome and cause random mutations within the target cell, thus limiting the use of these cells for downstream applications. To overcome this limitation, various non-integrating techniques, including Sendai virus, mRNA, minicircle, and plasmid-based methods, have recently been developed. Utilizing a newly developed codon optimized 4-in-1 minicircle (CoMiC), we were able to reprogram human adult fibroblasts using chemically defined media and without the need for feeder cells.
Keywords:	Human Induced Pluripotent Stem Cells (hiPSC), Minicircle, Reprogramming, Disease Modeling, Chemically Defined, Differentiation
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	Cardiac Tissue Engineering
ISSN:	1064-3745
ISBN:	978-1-4939-4613-6
Publisher:	Springer / Humana Press
Volume:	1181
Page Range:	1-13
Number of Pages:	266
Date:	1 January 2014
Official Publication:	https://doi.org/10.1007/978-1-4939-1047-2_1
PubMed:	View item in PubMed

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