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CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis

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Item Type:Article
Title:CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis
Creators Name:Müller, I. and Pappritz, K. and Savvatis, K. and Puhl, K. and Dong, F. and El-Shafeey, M. and Hamdani, N. and Hamann, I. and Noutsias, M. and Infante-Duarte, C. and Linke, W.A. and Van Linthout, S. and Tschöpe, C.
Abstract:Studies on inflammatory disorders elucidated the pivotal role of the CX3CL1/CX3CR1 axis with respect to the pathophysiology and diseases progression. Coxsackievirus B3 (CVB3)-induced myocarditis is associated with severe cardiac inflammation, which may progress to heart failure. We therefore investigated the influence of CX3CR1 ablation in the model of acute myocarditis, which was induced by inoculation with 5x10(5) plaque forming units of CVB3 (Nancy strain) in either CX3CR1(-/-) or C57BL6/j (WT) mice. Seven days after infection, myocardial inflammation, remodeling, and titin expression and phosphorylation were examined by immunohistochemistry, real-time PCR and Pro-Q diamond stain. Cardiac function was assessed by tip catheter. Compared to WT CVB3 mice, CX3CR1(-/-) CVB3 mice exhibited enhanced left ventricular expression of inflammatory cytokines and chemokines, which was associated with an increase of immune cell infiltration/presence. This shift towards a pro-inflammatory immune response further resulted in increased cardiac fibrosis and cardiomyocyte apoptosis, which was reflected by an impaired cardiac function in CX3CR1(-/-) CVB3 compared to WT CVB3 mice. These findings demonstrate a cardioprotective role of CX3CR1 in CVB3-infected mice and indicate the relevance of the CX3CL1/CX3CR1 system in CVB3-induced myocarditis.
Keywords:Animal Disease Models, Apoptosis, Cardiac Myocytes, Cell Adhesion Molecules, Chemokine CX3CL1, Chemokine Receptors, Coxsackievirus Infections, CX3C Chemokine Receptor 1, Gene Expression Regulation, Heart Function Tests, Host-Pathogen Interactions, Human Enterovirus B, Inbred C57BL Mice, Interleukins, Knockout Mice, Myocarditis, Phosphorylation, Protein Kinases, Animals, Mice
Source:PLoS ONE
Publisher:Public Library of Science
Page Range:e0182643
Date:11 August 2017
Official Publication:https://doi.org/10.1371/journal.pone.0182643
PubMed:View item in PubMed

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