A continuous intracerebral gene delivery system for in vivo liposome mediated gene therapy

Item Type:	Article
Title:	A continuous intracerebral gene delivery system for in vivo liposome mediated gene therapy
Creators Name:	Zhu, J. and Zhang, L. and Hanisch, U.K. and Felgner, P.L. and Reszka, R.
Abstract:	Using a minipump combined with stereotaxic techniques allows continuous delivery of therapeutic genetic materials into the brain. We investigated the therapeutic efficacy of liposome-mediated HSVtk gene transfer of experimental brain F98 glioma followed by treatment with ganciclovir. A single injection of DNA-liposome complexes showed a therapeutically significant decrease in the tumor volume. Continuous intracerebral delivery of DNA-liposome complexes using an osmotic minipump led to complete tumor regression in 36.4% of the treated animals. The safety and toxicity of this gene delivery system were also assessed. No organ pathology was observed in the experimental animals. The continuous gene delivery system could be a useful means of achieving higher doses with less toxicity and without the need for frequent injections.
Keywords:	In Vivo Gene Therapy, Cationic Liposome, Glioma, Thymidine Kinase, Continuous Delivery, Minipump, Animals, Rats
Source:	Gene Therapy
ISSN:	0969-7128
Publisher:	Nature Publishing Group
Volume:	3
Number:	6
Page Range:	472-476
Date:	June 1996
PubMed:	View item in PubMed

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