Generation of oligodendrocyte precursor cells from mouse dorsal spinal cord independent of Nkx6 regulation and Shh signaling

Item Type:	Article
Title:	Generation of oligodendrocyte precursor cells from mouse dorsal spinal cord independent of Nkx6 regulation and Shh signaling
Creators Name:	Cai, J. and Qi, Y. and Hu, X. and Tan, M. and Liu, Z. and Zhang, J. and Li, Q. and Sander, M. and Qiu, M.
Abstract:	In the developing spinal cord, early progenitor cells of the oligodendrocyte lineage are induced in the motor neuron progenitor (pMN) domain of the ventral neuroepithelium by the ventral midline signal Sonic hedgehog (Shh). The ventral generation of oligodendrocytes requires Nkx6-regulated expression of the bHLH gene Olig2 in this domain. In the absence of Nkx6 genes or Shh signaling, the initial expression of Olig2 in the pMN domain is completely abolished. In this study, we provide the in vivo evidence for a late phase of Olig gene expression independent of Nkx6 and Shh gene activities and reveal a brief second wave of oligodendrogenesis in the dorsal spinal cord. In addition, we provide genetic evidence that oligodendrogenesis can occur in the absence of hedgehog receptor Smoothened, which is essential for all hedgehog signaling.
Keywords:	Basic Helix-Loop-Helix Transcription Factors, Cell Differentiation, DNA-Binding Proteins, Epithelial Cells, Developmental Gene Expression Regulation, Hedgehog Proteins, Homeodomain Proteins, Immunohistochemistry, Nerve Tissue Proteins, Oligodendrocyte Transcription Factor 2, Oligodendroglia, PAX7 Transcription Factor, G-Protein-Coupled Receptors, Signal Transduction, Smoothened Receptor, Spinal Cord, Stem Cells, Trans-Activators, Transcription Factors / Metabolism, Animals, Mice
Source:	Neuron
ISSN:	0896-6273
Publisher:	Cell Press
Volume:	45
Number:	1
Page Range:	41-53
Date:	6 January 2005
Official Publication:	https://doi.org/10.1016/j.neuron.2004.12.028
PubMed:	View item in PubMed

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