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Teichoic acids anchor distinct cell wall lamellae in an apically growing bacterium

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Item Type:Article
Title:Teichoic acids anchor distinct cell wall lamellae in an apically growing bacterium
Creators Name:Ultee, E. and van der Aart, L.T. and Zhang, L. and van Dissel, D. and Diebolder, C.A. and van Wezel, G.P. and Claessen, D. and Briegel, A.
Abstract:The bacterial cell wall is a multicomponent structure that provides structural support and protection. In monoderm species, the cell wall is made up predominantly of peptidoglycan, teichoic acids and capsular glycans. Filamentous monoderm Actinobacteria incorporate new cell-wall material at their tips. Here we use cryo-electron tomography to reveal the architecture of the actinobacterial cell wall of Streptomyces coelicolor. Our data shows a density difference between the apex and subapical regions. Removal of teichoic acids results in a patchy cell wall and distinct lamellae. Knock-down of tagO expression using CRISPR-dCas9 interference leads to growth retardation, presumably because build-in of teichoic acids had become rate-limiting. Absence of extracellular glycans produced by MatAB and CslA proteins results in a thinner wall lacking lamellae and patches. We propose that the Streptomyces cell wall is composed of layers of peptidoglycan and extracellular polymers that are structurally supported by teichoic acids.
Keywords:Bacterial Proteins, CRISPR-Cas Systems, Cell Wall, Cryoelectron Microscopy, Bacterial Gene Expression Regulation, Peptidoglycan, Polysaccharides, Streptomyces coelicolor, Teichoic Acids, Tomography
Source:Communications Biology
Publisher:Springer Nature
Page Range:314
Date:17 June 2020
Official Publication:https://doi.org/10.1038/s42003-020-1038-6
PubMed:View item in PubMed

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