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3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells

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Item Type:Article
Title:3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells
Creators Name:Müller, A. and Schmidt, D. and Xu, C.S. and Pang, S. and D'Costa, J.V. and Kretschmar, S. and Münster, C. and Kurth, T. and Jug, F. and Weigert, M. and Hess, H.F. and Solimena, M.
Abstract:Microtubules play a major role in intracellular trafficking of vesicles in endocrine cells. Detailed knowledge of microtubule organization and their relation to other cell constituents is crucial for understanding cell function. However, their role in insulin transport and secretion is under debate. Here, we use FIB-SEM to image islet β cells in their entirety with unprecedented resolution. We reconstruct mitochondria, Golgi apparati, centrioles, insulin secretory granules, and microtubules of seven β cells, and generate a comprehensive spatial map of microtubule-organelle interactions. We find that microtubules form nonradial networks that are predominantly not connected to either centrioles or endomembranes. Microtubule number and length, but not microtubule polymer density, vary with glucose stimulation. Furthermore, insulin secretory granules are enriched near the plasma membrane, where they associate with microtubules. In summary, we provide the first 3D reconstructions of complete microtubule networks in primary mammalian cells together with evidence regarding their importance for insulin secretory granule positioning and thus their supportive role in insulin secretion.
Keywords:Cell Membrane, Cell Nucleus, Cultured Cells, Glucose, Three-Dimensional Imaging, Insulin, Insulin-Secreting Cells, Inbred C57BL Mice, Scanning Electron Microscopy, Microtubules, Organelles, Secretory Vesicles, Animals, Mice
Source:Journal of Cell Biology
Publisher:Rockefeller University Press
Page Range:e202010039
Date:1 February 2021
Official Publication:https://doi.org/10.1083/jcb.202010039
PubMed:View item in PubMed

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