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Different properties of caffeine-sensitive Ca2+ stores in peripheral and central mammalian neurones

Item Type:Article
Title:Different properties of caffeine-sensitive Ca2+ stores in peripheral and central mammalian neurones
Creators Name:Shmigol, A. and Kirischuk, S. and Kostyuk, P. and Verkhratsky, A.
Abstract:Using indo-1 based microfluorometry for measuring the cytoplasmic free calcium concentration ([Ca2+]i), the properties of caffeine-induced Ca2+ release from internal stores were studied in rat cultured central and peripheral neurones, including dorsal root ganglia (DRG) neurones, neurones from nucleus cuneatus, CA1 and CA3 hippocampal region and pyramidal neocortical neurones. Under resting conditions the Ca2+ content of internal stores in DRG neurones was high enough to produce caffeine-triggered [Ca2+]i transients. Caffeine-induced Ca2+ release depleted internal stores in DRG neurones, but they refilled themselves spontaneously up to 81.4 +/- 5.67% within 10 minutes. In contrast, in all types of central neurones the resting Ca2+ content of internal stores was low, but the stores could be charged by transmembrane Ca2+ influx through voltage-operated calcium channels. After charging, the stores in central neurones spontaneously lost releasable calcium content and within 10 minutes they emptied again. We suggest that in sensory neurones calcium stores are continuously filled by releasable calcium and after discharge they can refill themselves spontaneously, while in central neurones internal calcium stores can be charged by releasable calcium only transiently.
Keywords:Neurones, Cytoplasmic Calcium, Calcium Stores, Caffeine
Source:Pfluegers Archiv European Journal of Physiology
ISSN:0031-6768
Publisher:Springer (Germany)
Volume:426
Number:1-2
Page Range:174-176
Date:1 January 1994
Official Publication:https://doi.org/10.1007/BF00374686
PubMed:View item in PubMed

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