Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

A member of the tryptophan-rich protein family is required for efficient sequestration of Plasmodium berghei schizonts

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
2MB
[thumbnail of Supporting Information] Other (Supporting Information)
325kB

Item Type:Article
Title:A member of the tryptophan-rich protein family is required for efficient sequestration of Plasmodium berghei schizonts
Creators Name:Gabelich, J.A., Grützke, J., Kirscht, F., Popp, O., Matz, J.M., Dittmar, G., Rug, M. and Ingmundson, A.
Abstract:Protein export and host membrane remodeling are crucial for multiple Plasmodium species to establish a niche in infected hosts. To better understand the contribution of these processes to successful parasite infection in vivo, we sought to find and characterize protein components of the intraerythrocytic Plasmodium berghei-induced membrane structures (IBIS) that form in the cytoplasm of infected erythrocytes. We identified proteins that immunoprecipitate with IBIS1, a signature member of the IBIS in P. berghei-infected erythrocytes. In parallel, we also report our data describing proteins that co-precipitate with the PTEX (Plasmodium translocon of exported proteins) component EXP2. To validate our findings, we examined the location of three candidate IBIS1-interactors that are conserved across multiple Plasmodium species, and we found they localized to IBIS in infected red blood cells and two further colocalized with IBIS1 in the liver-stage parasitophorous vacuole membrane. Successful gene deletion revealed that these two tryptophan-rich domain-containing proteins, termed here IPIS2 and IPIS3 (for intraerythrocytic Plasmodium-induced membrane structures), are required for efficient blood-stage growth. Erythrocytes infected with IPIS2-deficient schizonts in particular fail to bind CD36 as efficiently as wild-type P. berghei-infected cells and therefore fail to effectively sequester out of the circulating blood. Our findings support the idea that intra-erythrocytic membrane compartments are required across species for alterations of the host erythrocyte that facilitate interactions of infected cells with host tissues.
Keywords:Erythrocytes, Protein Transport, Protozoan Proteins, Schizonts, Tryptophan, Animals, Plasmodium berghei, Plasmodium falciparum
Source:PLoS Pathogens
ISSN:1553-7366
Publisher:Public Library of Science
Volume:18
Number:9
Page Range:e1010846
Date:20 September 2022
Official Publication:https://doi.org/10.1371/journal.ppat.1010846
PubMed:View item in PubMed
Related to:

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library