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Branch point evolution controls species-specific alternative splicing and regulates long term potentiation

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Title:Branch point evolution controls species-specific alternative splicing and regulates long term potentiation
Creators Name:Franz, A. and Weber, A.I. and Preußner, M. and Dimos, N. and Stumpf, A. and Ji, Y. and Moreno-Velasquez, L. and Voigt, A. and Schulz, F. and Neumann, A. and Kuropka, B. and Kühn, R. and Urlaub, H. and Schmitz, D. and Wahl, M.C. and Heyd, F.
Abstract:Regulation and functionality of species-specific alternative splicing has remained enigmatic to the present date. Calcium/calmodulin-dependent protein kinase IIβ (CaMKIIβ) is expressed in several splice variants and plays a key role in learning and memory. Here, we identify and characterize several primate-specific CAMK2B splice isoforms, which show altered kinetic properties and changes in substrate specificity. Furthermore, we demonstrate that primate-specific Camk2β alternative splicing is achieved through branch point weakening during evolution. We show that reducing branch point and splice site strengths during evolution globally renders constitutive exons alternative, thus providing a paradigm for cis-directed species-specific alternative splicing regulation. Using CRISPR/Cas9 we introduced a weaker human branch point into the mouse genome, resulting in human-like CAMK2B splicing in the brain of mutant mice. We observe a strong impairment of long-term potentiation in CA3-CA1 synapses of mutant mice, thus connecting branch point-controlled, species-specific alternative splicing with a fundamental function in learning and memory.
Keywords:Animals, Mice
Publisher:Cold Spring Harbor Laboratory Press
Article Number:2022.09.09.507289
Date:11 September 2022
Official Publication:https://doi.org/10.1101/2022.09.09.507289
Related to:
https://edoc.mdc-berlin.de/22988/Final version

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