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Role of endothelial kinin B(1) receptor on the membrane potential of transgenic rat aorta

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Item Type:Article
Title:Role of endothelial kinin B(1) receptor on the membrane potential of transgenic rat aorta
Creators Name:Batista, C. and Sales, V.M. and Merino, V.F. and Bader, M. and Feres, T. and Pesquero, J.B.
Abstract:The kinin receptors are classically involved in inflammation, pain and sepsis. The effects of the kinin B(1) receptor agonist des-Arg(9)-bradykinin (DBK) and lipopolysaccharide (LPS) were investigated by comparing the membrane potential responses of aortic rings from transgenic rats overexpressing the kinin B1 receptor (B1R) in the endothelium (TGR(Tie2B1)) and Sprague Dawley (SD) rats. No difference in the resting membrane potential in the aorta's smooth muscle from the transgenic and SD rats was observed. The aorta rings from SD rats hyperpolarized only to LPS but not to DBK, whereas the aorta rings from TGR(Tie2B(1)) responded by the administration of both drugs. DBK and LPS responses were inhibited by the B(1) receptor antagonist R715 and by iberiotoxin in both cases. Thapsigargin induced a hyperpolarization in the smooth muscle of SD rats that was not reversed by R715, but was reversed by iberiotoxin and this hyperpolarization was further augmented by DBK administration. These results show that the model of overexpression of vascular B(1) receptors in the TGR(Tie2B(1)) rats represent a good model to study the role of functional B(1) receptors in the absence of any pathological stimulus. The data also show that K(Ca) channels are the final mediators of the hyperpolarizing responses to DBK and LPS. In addition, we suggest an interaction between the B1R and TLR4, since the hyperpolarization induced by LPS could be abolished in the presence of R715.
Keywords:TGR(Tie2B(1)) Transgenic Rat, Kinin B(1) Receptors, Potassium Channels, Membrane Potential, LPS, Animals, Rats
Source:Physiological Research
Publisher:Institute of Physiology, Czech Academy of Sciences
Page Range:477-487
Date:August 2022
Official Publication:http://www.biomed.cas.cz/physiolres/pdf/prepress/934904.pdf
PubMed:View item in PubMed

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