Helmholtz Gemeinschaft


Protein abundance control by non-coding antisense transcription

PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
[img] Other (Supplemental Information)

Item Type:Article
Title:Protein abundance control by non-coding antisense transcription
Creators Name:Huber, F. and Bunina, D. and Gupta, I. and Khmelinskii, A. and Meurer, M. and Theer, P. and Steinmetz, L.M. and Knop, M.
Abstract:Stable unannotated transcripts (SUTs), some of which overlap protein-coding genes in antisense direction, are a class of non-coding RNAs. While case studies have reported important regulatory roles for several of such RNAs, their general impact on protein abundance regulation of the overlapping gene is not known. To test this, we employed seamless gene manipulation to repress antisense SUTs of 162 yeast genes by using a unidirectional transcriptional terminator and a GFP tag. We found that the mere presence of antisense SUTs was not sufficient to influence protein abundance, that observed effects of antisense SUTs correlated with sense transcript start site overlap, and that the effects were generally weak and led to reduced protein levels. Antisense regulated genes showed increased H3K4 di- and trimethylation and had slightly lower than expected noise levels. Our results suggest that the functionality of antisense RNAs has gene and condition-specific components.
Keywords:Antisense RNA, Base Sequence, Fungal Gene Expression Regulation, Fungal RNA, Gene Library, Genetic Transcription, Histones, Lysine, Methylation, Microscopy, Proteins, Reproducibility of Results, Saccharomyces cerevisiae, Transcription Initiation Site, Untranslated RNA
Source:Cell Reports
Publisher:Cell Press / Elsevier
Page Range:2625-2636
Date:21 June 2016
Official Publication:https://doi.org/10.1016/j.celrep.2016.05.043
PubMed:View item in PubMed

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library