Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities

[img]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
4MB
[img]
Preview
PDF (Supplementary Data) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB

Item Type:Article
Title:Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities
Creators Name:Bunina, D. and Štefl, M. and Huber, F. and Khmelinskii, A. and Meurer, M. and Barry, J.D. and Kats, I. and Kirrmaier, D. and Huber, W. and Knop, M.
Abstract:Pervasive transcription of genomes generates multiple classes of non-coding RNAs. One of these classes are stable long non-coding RNAs which overlap coding genes in antisense direction (asRNAs). The function of such asRNAs is not fully understood but several cases of antisense-dependent gene expression regulation affecting the overlapping genes have been demonstrated. Using high-throughput yeast genetics and a limited set of four growth conditions we previously reported a regulatory function for ∼25% of asRNAs, most of which repress the expression of the sense gene. To further explore the roles of asRNAs we tested more conditions and identified 15 conditionally antisense-regulated genes, 6 of which exhibited antisense-dependent enhancement of gene expression. We focused on the sporulation-specific gene SPS100, which becomes upregulated upon entry into starvation or sporulation as a function of the antisense transcript SUT169. We demonstrate that the antisense effect is mediated by its 3' intergenic region (3'-IGR) and that this regulation can be transferred to other genes. Genetic analysis revealed that SUT169 functions by changing the relative expression of SPS100 mRNA isoforms from a short and unstable transcript to a long and stable species. These results suggest a novel mechanism of antisense-dependent gene regulation via mRNA isoform switching.
Keywords:Antisense RNA, Fluorescence Microscopy, Fungal Gene Expression Regulation, Fungal Spores, Immunoblotting, RNA Isoforms, RNA Stability, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time-Lapse Imaging, Up-Regulation
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press
Volume:45
Number:19
Page Range:11144-11158
Date:2 November 2017
Official Publication:https://doi.org/10.1093/nar/gkx737
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library