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NSun2-mediated cytosine-5 methylation of vault noncoding RNA determines its processing into regulatory small RNAs

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Item Type:Article
Title:NSun2-mediated cytosine-5 methylation of vault noncoding RNA determines its processing into regulatory small RNAs
Creators Name:Hussain, S. and Sajini, A.A. and Blanco, S. and Dietmann, S. and Lombard, P. and Sugimoto, Y. and Paramor, M. and Gleeson, J.G. and Odom, D.T. and Ule, J. and Frye, M.
Abstract:Autosomal-recessive loss of the NSUN2 gene has been identified as a causative link to intellectual disability disorders in humans. NSun2 is an RNA methyltransferase modifying cytosine-5 in transfer RNAs (tRNAs), yet the identification of cytosine methylation in other RNA species has been hampered by the lack of sensitive and reliable molecular techniques. Here, we describe miCLIP as an additional approach for identifying RNA methylation sites in transcriptomes. miCLIP is a customized version of the individual-nucleotide-resolution crosslinking and immunoprecipitation (iCLIP) method. We confirm site-specific methylation in tRNAs and additional messenger and noncoding RNAs (ncRNAs). Among these, vault ncRNAs contained six NSun2-methylated cytosines, three of which were confirmed by RNA bisulfite sequencing. Using patient cells lacking the NSun2 protein, we further show that loss of cytosine-5 methylation in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Thus, impaired processing of vault ncRNA may contribute to the etiology of NSun2-deficiency human disorders.
Keywords:Base Sequence, COS Cell, Cytosine, DNA Methylation, HEK293 Cells, Methyltransferases, Molecular Sequence Data, RNA, Transcriptome, Transfection, Untranslated RNA, Animals, Chlorocebus aethiops
Source:Cell Reports
Publisher:Cell Press / Elsevier
Page Range:255-261
Date:25 July 2013
Official Publication:https://doi.org/10.1016/j.celrep.2013.06.029
PubMed:View item in PubMed

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