Comprehensive identification of plant polyphenols by LC-MS

Item Type:	Book Section
Title:	Comprehensive identification of plant polyphenols by LC-MS
Creators Name:	Fernández-Ochoa, Á. and de la Luz Cádiz-Gurrea, M. and Segura Carretero, A.
Abstract:	The study of plant phenolic compounds has increased during the last decades due to their bioactive properties as well as their structural variability. This advance has been possible thanks to the development of advanced analytical techniques such as mass spectrometry, chromatographic techniques, and nuclear magnetic resonance. In addition, the development of software and databases has played a fundamental role in advancing the chemical characterization of these compounds. In this chapter, we present a protocol for the comprehensive identification of phenolic compounds based on liquid chromatography (reverse-phase mode) coupled to high-resolution mass spectrometry, which has been the most widely used technique to study this type of compounds. Furthermore, a data processing methodology based on open software and databases is detailed in this protocol.
Keywords:	Polyphenols, Phenolic Compounds, Mass Spectrometry, Liquid Chromatography, Characterization, Identification, Bioactive Compounds, Open-Source Software
Source:	Methods and Protocols in Food Science
Series Name:	Methods and Protocols in Food Science
Title of Book:	Mass spectrometry for food analysis
ISSN:	2662-950X
ISBN:	978-1-0716-2106-6
Publisher:	Humana
Page Range:	31-42
Date:	26 June 2022
Official Publication:	https://doi.org/10.1007/978-1-0716-2107-3_4

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