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Generation of redirected engineered human chimeric antigen receptor (CAR) T cells

Item Type:Book Section
Title:Generation of redirected engineered human chimeric antigen receptor (CAR) T cells
Creators Name:Bunse, M. and Höpken, U.E.
Abstract:Chimeric antigen receptor (CAR) T cell therapy that involves genetic engineering a patient's own immune cells with antigen-specific receptors has shown remarkable efficacy in blood cancer treatment. Numerous clinical studies with CAR T cells targeting the blood cell surface protein CD19 led to the FDA 's first approval of a genetically engineered cell therapy. The process of generating potent CAR T cells involves several carefully performed manufacturing steps. Here, we describe the generation of redirected engineered human CAR T cells for preclinical studies starting with the CAR design, retroviral gene transfer, detection of CAR expression, and expansion of transduced T cells.
Keywords:Chimeric Antigen Receptor, T Cells, Retroviral Transduction, Tumor Immunotherapy
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Gene therapy of cancer : methods and protocols
ISSN:1064-3745
ISBN:9781071624401
Publisher:Springer / Humana Press
Volume:2521
Number:3
Page Range:67-83
Number of Pages:0
Date:23 June 2022
Official Publication:https://doi.org/10.1007/978-1-0716-2441-8_4
PubMed:View item in PubMed
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https://edoc.mdc-berlin.de/21792/Book

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