Helmholtz Gemeinschaft


The impact of membrane protein diffusion on GPCR signaling

PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader

Item Type:Article
Title:The impact of membrane protein diffusion on GPCR signaling
Creators Name:Boltz, H.H. and Sirbu, A. and Stelzer, N. and de Lanerolle, P. and Winkelmann, S. and Annibale, P.
Abstract:Spatiotemporal signal shaping in G protein-coupled receptor (GPCR) signaling is now a well-established and accepted notion to explain how signaling specificity can be achieved by a superfamily sharing only a handful of downstream second messengers. Dozens of Gs-coupled GPCR signals ultimately converge on the production of cAMP, a ubiquitous second messenger. This idea is almost always framed in terms of local concentrations, the differences in which are maintained by means of spatial separation. However, given the dynamic nature of the reaction-diffusion processes at hand, the dynamics, in particular the local diffusional properties of the receptors and their cognate G proteins, are also important. By combining some first principle considerations, simulated data, and experimental data of the receptors diffusing on the membranes of living cells, we offer a short perspective on the modulatory role of local membrane diffusion in regulating GPCR-mediated cell signaling. Our analysis points to a diffusion-limited regime where the effective production rate of activated G protein scales linearly with the receptor-G protein complex's relative diffusion rate and to an interesting role played by the membrane geometry in modulating the efficiency of coupling.
Keywords:GPCR Signaling, Spatiotemporal Signal Shaping, Diffusion-Limited Reaction
Page Range:1660
Date:17 May 2022
Official Publication:https://doi.org/10.3390/cells11101660
PubMed:View item in PubMed

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library