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| Item Type: | Article |
|---|---|
| Title: | The anti-glucocorticoid receptor antibody clone 5E4: raising awareness of unspecific antibody binding |
| Creators Name: | Ehlers, L. and Kirchner, M. and Mertins, P. and Strehl, C. and Buttgereit, F. and Gaber, T. |
| Abstract: | Unspecific antibody binding takes a significant toll on researchers in the form of both the economic burden and the disappointed hopes of promising new therapeutic targets. Despite recent initiatives promoting antibody validation, a uniform approach addressing this issue has not yet been developed. Here, we demonstrate that the anti-glucocorticoid receptor (GR) antibody clone 5E4 predominantly targets two different proteins of approximately the same size, namely AMP deaminase 2 (AMPD2) and transcription intermediary factor 1-beta (TRIM28). This paper is intended to generate awareness of unspecific binding of well-established reagents and advocate the use of more rigorous verification methods to improve antibody quality in the future. |
| Keywords: | Antibody Specificity, Cross-Reactivity, Replication Crisis, Glucocorticoid Receptor |
| Source: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Publisher: | MDPI |
| Volume: | 23 |
| Number: | 9 |
| Page Range: | 5049 |
| Date: | 2 May 2022 |
| Official Publication: | https://doi.org/10.3390/ijms23095049 |
| PubMed: | View item in PubMed |
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