Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry

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Item Type:	Article
Title:	Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry
Creators Name:	Menzel, L., Zschummel, M. and Rehm, A.
Abstract:	Blood endothelial cells (BECs) in lymph nodes are distinct stromal cells with a transcriptional profile allowing fast and specific adaptation to the functional requirements. Here, we describe a step-by-step protocol for the enzymatic digestion of lymph nodes, the enrichment of stromal cells, the sorting of BECs, and the processing of BEC-related data for modern analysis approaches as spectral flow cytometry and single-cell RNA sequencing (scRNA-seq). For complete details on the use and execution of this protocol, please refer to Menzel et al. (2021).
Keywords:	Bioinformatics, Cell Isolation, Single Cell, Flow Cytometry/Mass Cytometry, Cancer, Genomics, RNAseq, Immunology, Animals, Mice
Source:	STAR Protocols
ISSN:	2666-1667
Publisher:	Elsevier
Volume:	3
Number:	2
Page Range:	101267
Date:	17 June 2022
Official Publication:	https://doi.org/10.1016/j.xpro.2022.101267
PubMed:	View item in PubMed

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