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Unraveling the hidden temporal range of fast β(2)-adrenergic receptor mobility by time-resolved fluorescence

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Item Type:Article
Title:Unraveling the hidden temporal range of fast β(2)-adrenergic receptor mobility by time-resolved fluorescence
Creators Name:Balakrishnan, A. and Hemmen, K. and Choudhury, S. and Krohn, J.H. and Jansen, K. and Friedrich, M. and Beliu, G. and Sauer, M. and Lohse, M.J. and Heinze, K.G.
Abstract:G-protein-coupled receptors (GPCRs) are hypothesized to possess molecular mobility over a wide temporal range. Until now the temporal range has not been fully accessible due to the crucially limited temporal range of available methods. This in turn, may lead relevant dynamic constants to remain masked. Here, we expand this dynamic range by combining fluorescent techniques using a spot confocal setup. We decipher mobility constants of β(2)-adrenergic receptor over a wide time range (nanosecond to second). Particularly, a translational mobility (10 µm(2)/s), one order of magnitude faster than membrane associated lateral mobility that explains membrane protein turnover and suggests a wider picture of the GPCR availability on the plasma membrane. And a so far elusive rotational mobility (1-200 µs) which depicts a previously overlooked dynamic component that, despite all complexity, behaves largely as predicted by the Saffman-Delbrück model.
Keywords:Cell Membrane, Membrane Proteins, Signal Transduction, Fluorescence Spectrometry
Source:Communications Biology
ISSN:2399-3642
Publisher:Springer Nature
Volume:5
Number:1
Page Range:176
Date:28 February 2022
Official Publication:https://doi.org/10.1038/s42003-022-03106-4
PubMed:View item in PubMed

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