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Expression of the chemokine receptor BLR2/EBI1 is specifically transactivated by Epstein-Barr Virus nuclear antigen 2

Item Type:Article
Title:Expression of the chemokine receptor BLR2/EBI1 is specifically transactivated by Epstein-Barr Virus nuclear antigen 2
Creators Name:Burgstahler, R. and Kempkes, B. and Steube, K. and Lipp, M.
Abstract:In our attempt to identify chemokine receptors that are related to Burkitt's lymphoma receptor 1 (BLR1) and are expressed in activated lymphocytes we used RT-PCR resulting in the isolation of a cDNA encoding a seven transmembrane receptor termed BLR2. The protein shows significant sequence similarities to the family of G-protein coupled chemokine receptors and turned out to be identical to the recently described receptor EBI1. Northern blot analysis revealed that BLR2 mRNA could be highly stimulated in mitogen- and anti-CD3-treated peripheral blood lymphocytes. BLR2-specific mRNA could be detected in all Epstein-Barr virus positive B cell lines. We show that transcription of the BLR2 gene could be specifically induced in Epstein-Barr virus negative BL 41 cells via estrogen-mediated activation of Epstein-Barr virus nuclear antigen 2, a key regulator of viral and cellular genes in immortalized B cells. Our data suggest an involvement of BLR2 in the regulation of migration in activated lymphocytes and in viral pathogenesis.
Keywords:B-Lymphocytes, Base Sequence, CCR7 Receptors, Cell Surface Receptors, Chemokine Receptors, Complementary DNA, Cultured Cells, DNA Primers, DNA-Binding Proteins, Epstein-Barr Virus Nuclear Antigens, Gene Expression, Genetic Transcription, Human Herpesvirus 4, Lymphocyte Activation, Lymphocytes, Messenger RNA, Molecular Cloning, Molecular Sequence Data, Polymerase Chain Reaction, Trans-Activators, Transcriptional Activation, Viral Antigens
Source:Biochemical and Biophysical Research Communications
Publisher:Academic Press
Page Range:737-743
Date:13 October 1995
Official Publication:https://doi.org/10.1006/bbrc.1995.2525
PubMed:View item in PubMed

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