Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain

Item Type:	Article
Title:	Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain
Creators Name:	Hamann, J. and Eichler, W. and Hamann, D. and Kerstens, H.M.J. and Poddighe, P.J. and Hoovers, J.M.N. and Hartmann, E. and Strauss, M. and Van Lier, R.A.W.
Abstract:	CD97 is a monomeric glycoprotein of 75 to 85 kDa that is induced rapidly on the surface of most leukocytes upon activation. We herein report the isolation of a cDNA encoding human CD97 by expression cloning in COS cells. The 3-kb cDNA clone encodes a mature polypeptide chain of 722 amino acids with a predicted molecular mass of 79 kDa. Within the C-terminal part of the protein, a region with seven hydrophobic segments was identified, suggesting that CD97 is a seven-span transmembrane molecule. Sequence comparison indicates that CD97 is the first leukocyte Ag in a recently described superfamily that includes the receptors for secretin, calcitonin, and other mammalian and insect peptide hormones. Different from these receptors, CD97 has an extended extracellular region of 433 amino acids that possesses three N-terminal epidermal growth factor-like domains, two of them with a calcium-binding site, and a single Arg-Gly-Asp (RGD) motif. The existence of structural elements characteristic for extracellular matrix proteins in a seven-span transmembrane molecule makes CD97 a receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. The gene encoding CD97 is localized on chromosome 19 (19p13.12-13.2).
Keywords:	Amino Acid Sequence, Base Sequence, CD Antigens, Chromosome Mapping, Complementary DNA, Cultured Cells, G-Protein-Coupled Receptors, Gastrointestinal Hormone Receptors, Gene Expression Regulation, Human Chromosomes Pair 19, Lymphocyte Activation, Membrane Glycoproteins, Molecular Cloning, Molecular Sequence Data
Source:	Journal of Immunology
ISSN:	0022-1767
Publisher:	American Association of Immunologists
Volume:	155
Number:	4
Page Range:	1942-1950
Date:	August 1995
Official Publication:	http://www.jimmunol.org/cgi/content/abstract/155/4/1942
PubMed:	View item in PubMed

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