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Light sheet fluorescence microscopy

Item Type:Review
Title:Light sheet fluorescence microscopy
Creators Name:Stelzer, E.H.K. and Strobl, F. and Chang, B.J. and Preusser, F. and Preibisch, S. and McDole, K. and Fiolka, R.
Abstract:Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical sectioning capability and, hence, provide axial resolution, restrict photobleaching and phototoxicity to a fraction of the sample and use cameras to record tens to thousands of images per second. LSMs are used for in-depth analyses of large, optically cleared samples and long-term three-dimensional (3D) observations of live biological specimens at high spatio-temporal resolution. The independently operated illumination and detection trains and the canonical implementations, selective/single plane illumination microscope (SPIM) and digital scanned laser microscope (DSLM), are the basis for many LSM designs. In this Primer, we discuss various applications of LSFM for imaging multicellular specimens, developing vertebrate and invertebrate embryos, brain and heart function, 3D cell culture models, single cells, tissue sections, plants, organismic interaction and entire cleared brains. Further, we describe the combination of LSFM with other imaging approaches to allow for super-resolution or increased penetration depth and the use of sophisticated spatio-temporal manipulations to allow for observations along multiple directions. Finally, we anticipate developments of the field in the near future.
Source:Nature Reviews Methods Primers
Publisher:Nature Research
Page Range:73
Date:3 November 2021
Official Publication:https://doi.org/10.1038/s43586-021-00069-4

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