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Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC

Item Type:Article
Title:Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC
Creators Name:Fiskin, E. and Lareau, C.A. and Ludwig, L.S. and Eraslan, G. and Liu, F. and Ring, A.M. and Xavier, R.J. and Regev, A.
Abstract:Multimodal measurements of single-cell profiles are proving increasingly useful for characterizing cell states and regulatory mechanisms. In the present study, we developed PHAGE-ATAC (Assay for Transposase-Accessible Chromatin), a massively parallel droplet-based method that uses phage displaying, engineered, camelid single-domain antibodies ('nanobodies') for simultaneous single-cell measurements of protein levels and chromatin accessibility profiles, and mitochondrial DNA-based clonal tracing. We use PHAGE-ATAC for multimodal analysis in primary human immune cells, sample multiplexing, intracellular protein analysis and the detection of SARS-CoV-2 spike protein in human cell populations. Finally, we construct a synthetic high-complexity phage library for selection of antigen-specific nanobodies that bind cells of particular molecular profiles, opening an avenue for protein detection, cell characterization and screening with single-cell genomics.
Keywords:Gene Expression Analysis, Gene Regulation in Immune Cells, Genomics, Proteomics, Sequencing, Animals, Mice
Source:Nature Biotechnology
Publisher:Nature Publishing Group
Page Range:374-381
Date:March 2022
Additional Information:Copyright © 2021, The Author(s), under exclusive licence to Springer Nature America, Inc.
Official Publication:https://doi.org/10.1038/s41587-021-01065-5
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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