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| Item Type: | Article |
|---|---|
| Title: | RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis |
| Creators Name: | Zhang, S. and Übelmesser, N. and Josipovic, N. and Forte, G. and Slotman, J.A. and Chiang, M. and Gothe, H.J. and Gusmao, E.G. and Becker, C. and Altmüller, J. and Houtsmuller, A.B. and Roukos, V. and Wendt, K.S. and Marenduzzo, D. and Papantonis, A. |
| Abstract: | Mammalian chromosomes are three-dimensional entities shaped by converging and opposing forces. Mitotic cell division induces marked chromosome condensation, but following reentry into the G1 phase of the cell cycle, chromosomes reestablish their interphase organization. Here, we tested the role of RNA polymerase II (RNAPII) in this transition using a cell line that allows its auxin-mediated degradation. In situ Hi-C showed that RNAPII is required for both compartment and loop establishment following mitosis. RNAPs often counteract loop extrusion, and in their absence, longer and more prominent loops arose. Evidence from chromatin binding, super-resolution imaging, and in silico modeling allude to these effects being a result of RNAPII-mediated cohesin loading upon G1 reentry. Our findings reconcile the role of RNAPII in gene expression with that in chromatin architecture. |
| Source: | Science Advances |
| ISSN: | 2375-2548 |
| Publisher: | American Association for the Advancement of Science |
| Volume: | 7 |
| Number: | 43 |
| Page Range: | eabg8205 |
| Date: | 22 October 2021 |
| Official Publication: | https://doi.org/10.1126/sciadv.abg8205 |
| PubMed: | View item in PubMed |
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