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A previously undescribed highly prevalent phage identified in a Danish Enteric Virome Catalog

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Item Type:Article
Title:A previously undescribed highly prevalent phage identified in a Danish Enteric Virome Catalog
Creators Name:Van Espen, L. and Bak, E.G. and Beller, L. and Close, L. and Deboutte, W. and Juel, H.B. and Nielsen, T. and Sinar, D. and De Coninck, L. and Frithioff-Bøjsøe, C. and Fonvig, C.E. and Jacobsen, S. and Kjærgaard, M. and Thiele, M. and Fullam, A. and Kuhn, M. and Holm, J.C. and Bork, P. and Krag, A. and Hansen, T. and Arumugam, M. and Matthijnssens, J.
Abstract:Gut viruses are important, yet often neglected, players in the complex human gut microbial ecosystem. Recently, the number of human gut virome studies has been increasing; however, we are still only scratching the surface of the immense viral diversity. In this study, 254 virus-enriched fecal metagenomes from 204 Danish subjects were used to generate the anish nteric irme atalog (DEVoC) containing 12,986 nonredundant viral scaffolds, of which the majority was previously undescribed, encoding 190,029 viral genes. The DEVoC was used to compare 91 healthy DEVoC gut viromes from children, adolescents, and adults that were used to create the DEVoC. Gut viromes of healthy Danish subjects were dominated by phages. While most phage genomes (PGs) only occurred in a single subject, indicating large virome individuality, 39 PGs were present in more than 10 healthy subjects. Among these 39 PGs, the prevalences of three PGs were associated with age. To further study the prevalence of these 39 prevalent PGs, 1,880 gut virome data sets of 27 studies from across the world were screened, revealing several age-, geography-, and disease-related prevalence patterns. Two PGs also showed a remarkably high prevalence worldwide - a crAss-like phage (20.6% prevalence), belonging to the tentative subfamily, and a previously undescribed circular temperate phage infecting Bacteroides dorei (14.4% prevalence), called LoVEphage because it encodes ots f iral lements. Due to the LoVEphage's high prevalence and novelty, public data sets in which the LoVEphage was detected were assembled, resulting in an additional 18 circular LoVEphage-like genomes (67.9 to 72.4 kb).
Keywords:Human Gut Virome, Virome Catalog, Healthy Gut Viromes, Phages
Publisher:American Society for Microbiology
Page Range:e00382-21
Date:26 October 2021
Official Publication:https://doi.org/10.1128/mSystems.00382-21
PubMed:View item in PubMed

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