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| Item Type: | Article | ||||
|---|---|---|---|---|---|
| Title: | Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis | ||||
| Creators Name: | Moreno-Layseca, P. and Jäntti, N.Z. and Godbole, R. and Sommer, C. and Jacquemet, G. and Al-Akhrass, H. and Conway, J.R.W. and Kronqvist, P. and Kallionpää, R.E. and Oliveira-Ferrer, L. and Cervero, P. and Linder, S. and Aepfelbacher, M. and Zauber, H. and Rae, J. and Parton, R.G. and Disanza, A. and Scita, G. and Mayor, S. and Selbach, M. and Veltel, S. and Ivaska, J. | ||||
| Abstract: | Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery - Arf1, IRSp53 and actin - and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route. | ||||
| Keywords: | Actins, Biological Transport, Breast Neoplasms, Cell Movement, Clathrin, Dynamins, Endocytosis, Integrin beta1, Intracellular Signaling Peptides and Proteins, rab GTP-Binding Proteins | ||||
| Source: | Nature Cell Biology | ||||
| ISSN: | 1465-7392 | ||||
| Publisher: | Nature Publishing Group | ||||
| Volume: | 23 | ||||
| Number: | 10 | ||||
| Page Range: | 1073-1084 | ||||
| Date: | October 2021 | ||||
| Additional Information: | "Erratum in: Nat Cell Biol https://doi.org/10.1038/s41556-021-00767-x, published online 6 October 2021." | ||||
| Official Publication: | https://doi.org/10.1038/s41556-021-00767-x | ||||
| PubMed: | View item in PubMed | ||||
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