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A simple pressure-assisted method for MicroED specimen preparation

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Item Type:Article
Title:A simple pressure-assisted method for MicroED specimen preparation
Creators Name:Zhao, J. and Xu, H. and Lebrette, H. and Carroni, M. and Taberman, H. and Högbom, M. and Zou, X.
Abstract:Micro-crystal electron diffraction (MicroED) has shown great potential for structure determination of macromolecular crystals too small for X-ray diffraction. However, specimen preparation remains a major bottleneck. Here, we report a simple method for preparing MicroED specimens, named Preassis, in which excess liquid is removed through an EM grid with the assistance of pressure. We show the ice thicknesses can be controlled by tuning the pressure in combination with EM grids with appropriate carbon hole sizes. Importantly, Preassis can handle a wide range of protein crystals grown in various buffer conditions including those with high viscosity, as well as samples with low crystal concentrations. Preassis is a simple and universal method for MicroED specimen preparation, and will significantly broaden the applications of MicroED.
Source:Nature Communications
Publisher:Nature Publishing Group
Page Range:5036
Date:19 August 2021
Official Publication:https://doi.org/10.1038/s41467-021-25335-7
PubMed:View item in PubMed

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