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Place fields of single spikes in hippocampus involve Kcnq3 channel-dependent entrainment of complex spike bursts

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Item Type:Article
Title:Place fields of single spikes in hippocampus involve Kcnq3 channel-dependent entrainment of complex spike bursts
Creators Name:Gao, X. and Bender, F. and Soh, H. and Chen, C. and Altafi, M. and Schütze, S. and Heidenreich, M. and Gorbati, M. and Corbu, M.A. and Carus-Cadavieco, M. and Korotkova, T. and Tzingounis, A.V. and Jentsch, T.J. and Ponomarenko, A.
Abstract:Hippocampal pyramidal cells encode an animal’s location by single action potentials and complex spike bursts. These elementary signals are believed to play distinct roles in memory consolidation. The timing of single spikes and bursts is determined by intrinsic excitability and theta oscillations (5–10 Hz). Yet contributions of these dynamics to place fields remain elusive due to the lack of methods for specific modification of burst discharge. In mice lacking Kcnq3-containing M-type K(+) channels, we find that pyramidal cell bursts are less coordinated by the theta rhythm than in controls during spatial navigation, but not alert immobility. Less modulated bursts are followed by an intact post-burst pause of single spike firing, resulting in a temporal discoordination of network oscillatory and intrinsic excitability. Place fields of single spikes in one- and two-dimensional environments are smaller in the mutant. Optogenetic manipulations of upstream signals reveal that neither medial septal GABA-ergic nor cholinergic inputs alone, but rather their joint activity, is required for entrainment of bursts. Our results suggest that altered representations by bursts and single spikes may contribute to deficits underlying cognitive disabilities associated with KCNQ3-mutations in humans.
Keywords:Action Potentials, Hippocampus, KCNQ3 Potassium Channel, Inbred C57BL Mice, Knockout Mice, Transgenic Mice, Optogenetics, Pyramidal Cells, Theta Rhythm / Physiology, Animals, Mice
Source:Nature Communications
Publisher:Nature Publishing Group
Page Range:4801
Date:10 August 2021
Official Publication:https://doi.org/10.1038/s41467-021-24805-2
PubMed:View item in PubMed

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