Enrichment of target sequences for next-generation sequencing applications in research and diagnostics

Item Type:	Article
Title:	Enrichment of target sequences for next-generation sequencing applications in research and diagnostics
Creators Name:	Altmüller, J. and Budde, B.S. and Nürnberg, P.
Abstract:	Targeted re-sequencing such as gene panel sequencing (GPS) has become very popular in medical genetics, both for research projects and in diagnostic settings. The technical principles of the different enrichment methods have been reviewed several times before; however, new enrichment products are constantly entering the market, and researchers are often puzzled about the requirement to take decisions about long-term commitments, both for the enrichment product and the sequencing technology. This review summarizes important considerations for the experimental design and provides helpful recommendations in choosing the best sequencing strategy for various research projects and diagnostic applications.
Keywords:	Gene Panel Sequencing, Target Enrichment
Source:	Biological Chemistry
ISSN:	1431-6730
Publisher:	de Gruyter
Volume:	395
Number:	2
Page Range:	231-237
Date:	1 February 2014
Official Publication:	https://doi.org/10.1515/hsz-2013-0199
PubMed:	View item in PubMed

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