Transcriptome analysis of murine olfactory sensory neurons during development using single cell RNA-Seq

Item Type:	Article
Title:	Transcriptome analysis of murine olfactory sensory neurons during development using single cell RNA-Seq
Creators Name:	Scholz, P. and Kalbe, B. and Jansen, F. and Altmüller, J. and Becker, C. and Mohrhardt, J. and Schreiner, B. and Gisselmann, G. and Hatt, H. and Osterloh, S.
Abstract:	Mammalian odor reception is achieved by highly specialized olfactory sensory neurons (OSNs) located in the nasal cavity. Despite their importance for the daily survival of most mammals, the gene expression and regulatory profiles of these single neurons are poorly understood. Here, we report the isolation of individual GFP-labeled OSNs from Olfr73-GFP mice at different developmental stages followed by Next Generation Sequencing, thereby analyzing the detailed transcriptome for the first time. We characterized the repertoire of olfactory receptors (ORs) and found that in addition to the highly and predominant detectable Olfr73, 20 additional ORs were stably detectable at lower transcript levels in adult mice. Additionally, OSNs collected from mice of earlier developmental stages did not show any stable OR patterns. However, more than one predominant OR per OSN was detectable.
Keywords:	Development, Olfaction, Olfactory Receptors, Single Cell, Transcriptome Analysis, Animals, Mice
Source:	Chemical Senses
ISSN:	0379-864X
Publisher:	Oxford University Press
Volume:	41
Number:	4
Page Range:	313-323
Date:	May 2016
Official Publication:	https://doi.org/10.1093/chemse/bjw003
PubMed:	View item in PubMed

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