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Mechanism suppressing H3K9 trimethylation in pluripotent stem cells and its demise by polyQ-expanded huntingtin mutations

Item Type:Article
Title:Mechanism suppressing H3K9 trimethylation in pluripotent stem cells and its demise by polyQ-expanded huntingtin mutations
Creators Name:Irmak, D. and Fatima, A. and Gutiérrez-Garcia, R. and Rinschen, M.M. and Wagle, P. and Altmüller, J. and Arrigoni, L. and Hummel, B. and Klein, C. and Frese, C.K. and Sawarkar, R. and Rada-Iglesias, A. and Vilchez, D.
Abstract:Pluripotent stem cells are invaluable resources to study development and disease, holding a great promise for regenerative medicine. Here we use human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) from patients with Huntington's disease (HD-iPSCs) to shed light into the normal function of huntingtin (HTT) and its demise in disease. We find that HTT binds ATF7IP, a regulator of the histone H3 methyltransferase SETDB1. HTT inhibits the interaction of the ATF7IP-SETDB1 complex with other heterochromatin regulators and transcriptional repressors, maintaining low levels of H3K9 trimethylation (H3K9me3) in hESCs. Loss of HTT promotes global increased H3K9me3 levels and enrichment of H3K9me3 marks at distinct genes, including transcriptional regulators of neuronal differentiation. Although these genes are normally expressed at low amounts in hESCs, HTT knockdown (KD) reduces their induction during neural differentiation. Notably, mutant expanded polyglutamine repeats in HTT diminish its interaction with ATF7IP-SETDB1 complex and trigger H3K9me3 in HD-iPSCs. Conversely, KD of ATF7IP in HD-iPSCs reduces H3K9me3 alterations and ameliorates gene expression changes in their neural counterparts. Taken together, our results indicate ATF7IP as a potential target to correct aberrant H3K9me3 levels induced by mutant HTT.
Keywords:Developmental Gene Expression Regulation, Embryonic Stem Cells, Gene Knockdown Techniques, Heterochromatin, Histone Methyltransferases, Histone-Lysine N-Methyltransferase, Huntingtin Protein, Huntington Disease, Induced Pluripotent Stem Cells, Lentivirus, Neurons, Peptides, Protein Methyltransferases, Repressor Proteins, Transcription Factors
Source:Human Molecular Genetics
Publisher:Oxford University Press
Page Range:4117-4134
Date:1 December 2018
Official Publication:https://doi.org/10.1093/hmg/ddy304
PubMed:View item in PubMed

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