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cfNOMe - a single assay for comprehensive epigenetic analyses of cell-free DNA

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Item Type:Article
Title:cfNOMe - a single assay for comprehensive epigenetic analyses of cell-free DNA
Creators Name:Erger, F. and Nörling, D. and Borchert, D. and Leenen, E. and Habbig, S. and Wiesener, M.S. and Bartram, M.P. and Wenzel, A. and Becker, C. and Toliat, M.R. and Nürnberg, P. and Beck, B.B. and Altmüller, J.
Abstract:Cell-free DNA (cfDNA) analysis has become essential in cancer diagnostics and prenatal testing. We present cfNOMe, a two-in-one method of measuring cfDNA cytosine methylation and nucleosome occupancy in a single assay using non-disruptive enzymatic cytosine conversion and a custom bioinformatic pipeline. We show that enzymatic cytosine conversion better preserves cfDNA fragmentation information than does bisulfite conversion. Whereas previously separate experiments were required to study either epigenetic marking, cfNOMe delivers reliable results for both, enabling more comprehensive and inexpensive epigenetic cfDNA profiling. cfNOMe has the potential to advance biomarker discovery and diagnostic usage in diseases with systemic perturbations of cfDNA composition.
Keywords:cfNOMe, Cell-Free DNA, Methylome, Nucleosome Footprints, Analysis Software
Source:Genome Medicine
Publisher:BioMed Central
Page Range:54
Date:24 June 2020
Official Publication:https://doi.org/10.1186/s13073-020-00750-5
PubMed:View item in PubMed

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