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CRISPR-based diagnostics

Item Type:Review
Title:CRISPR-based diagnostics
Creators Name:Kaminski, M.M. and Abudayyeh, O.O. and Gootenberg, J.S. and Zhang, F. and Collins, J.J.
Abstract:The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel. Recent developments in diagnostic technologies, in particular those leveraging clustered regularly interspaced short palindromic repeats (CRISPR), aim to enable accurate testing at home, at the point of care and in the field. In this Review, we provide a rundown of the rapidly expanding toolbox for CRISPR-based diagnostics, in particular the various assays, preamplification strategies and readouts, and highlight their main applications in the sensing of a wide range of molecular targets relevant to human health.
Keywords:CRISPR-Cas Systems, Communicable Diseases, Inborn Genetic Diseases, Nucleic Acid Amplification Techniques, Nucleic Acids, Point-of-Care Systems, Single Nucleotide Polymorphism, DNA Sequence Analysis
Source:Nature Biomedical Engineering
ISSN:2157-846X
Publisher:Nature Publishing Group
Volume:5
Number:7
Page Range:643-656
Date:July 2021
Official Publication:https://doi.org/10.1038/s41551-021-00760-7
PubMed:View item in PubMed

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