Interaction of complexes I, III, and IV within the bovine respirasome by single particle cryoelectron tomography

Item Type:	Article
Title:	Interaction of complexes I, III, and IV within the bovine respirasome by single particle cryoelectron tomography
Creators Name:	Dudkina, N.V. and Kudryashev, M. and Stahlberg, H. and Boekema, E.J.
Abstract:	The respirasome is a multisubunit supercomplex of the respiratory chain in mitochondria. Here we report the 3D reconstruction of the bovine heart respirasome, composed of dimeric complex III and single copies of complex I and IV, at about 2.2-nm resolution, determined by cryoelectron tomography and subvolume averaging. Fitting of X-ray structures of single complexes I, III(2), and IV with high fidelity allows interpretation of the model at the level of secondary structures and shows how the individual complexes interact within the respirasome. Surprisingly, the distance between cytochrome c binding sites of complexes III(2) and IV is about 10 nm. Modeling indicates a loose interaction between the three complexes and provides evidence that lipids are gluing them at the interfaces.
Keywords:	Electron Microscopy, Oxidative Phosphorylation, Animals, Cattle
Source:	Proceedings of the National Academy of Sciences of the United States of America
ISSN:	0027-8424
Publisher:	National Academy of Sciences
Volume:	108
Number:	37
Page Range:	15196-15200
Date:	13 September 2011
Official Publication:	https://doi.org/10.1073/pnas.1107819108
PubMed:	View item in PubMed

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