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Luminal particles within cellular microtubules

Item Type:Article
Title:Luminal particles within cellular microtubules
Creators Name:Garvalov, B.K. and Zuber, B. and Bouchet-Marquis, C. and Kudryashev, M. and Gruska, M. and Beck, M. and Leis, A. and Frischknecht, F. and Bradke, F. and Baumeister, W. and Dubochet, J. and Cyrklaff, M.
Abstract:The regulation of microtubule dynamics is attributed to microtubule-associated proteins that bind to the microtubule outer surface, but little is known about cellular components that may associate with the internal side of microtubules. We used cryoelectron tomography to investigate in a quantitative manner the three dimensional structure of microtubules in intact mammalian cells. We show that the lumen of microtubules in this native state is filled with discrete, globular particles with a diameter of 7 nm and spacings between 8 and 20 nm in neuronal cells. Cross-sectional views of microtubules confirm the presence of luminal material in vitreous sections of brain tissue. Most of the luminal particles had connections to the microtubule wall, as revealed in tomograms. A higher accumulation of particles was seen near the retracting plus ends of microtubules. The luminal particles were abundant in neurons, but were also observed in other cells, such as astrocytes and stem cells.
Keywords:Astrocytes, Axons, Cryoelectron Microscopy, Cultured Cells, Cytoplasmic Granules, HeLa Cells, Hippocampus, Microtubule-Associated Proteins, Microtubules, Neurites, Neurons, Newborn Animals, Organ Culture Techniques, Pluripotent Stem Cells, Animals, Mice, Rats
Source:Journal of Cell Biology
ISSN:0021-9525
Publisher:Rockefeller University Press
Volume:174
Number:6
Page Range:759-765
Date:11 September 2006
Official Publication:https://doi.org/10.1083/jcb.200606074
PubMed:View item in PubMed

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