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| Item Type: | Article |
|---|---|
| Title: | Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress |
| Creators Name: | Devos, S. and Van Putte, W. and Vitse, J. and Van Driessche, G. and Stremersch, S. and Van Den Broek, W. and Raemdonck, K. and Braeckmans, K. and Stahlberg, H. and Kudryashev, M. and Savvides, S.N. and Devreese, B. |
| Abstract: | Several bacterial species produce membrane vesicles (MVs) in response to antibiotic stress. However, the biogenesis and role of MVs in bacterial antibiotic resistance mechanisms have remained unclear. Here, we studied the effect of the fluoroquinolone ciprofloxacin on MV secretion by Stenotrophomonas maltophilia using a combination of electron microscopy and proteomic approaches. We found that in addition to the classical outer membrane vesicles (OMV), ciprofloxacin-stimulated cultures produced larger vesicles containing both outer and inner membranes termed outer-inner membrane vesicles (OIMV), and that such MVs are enriched with cytosolic proteins. Remarkably, OIMV were found to be decorated with filamentous structures identified as fimbriae. In addition, ciprofloxacin stress leads to the release of bacteriophages and phage tail-like particles. Prophage induction by ciprofloxacin has been linked to pathogenesis and horizontal gene transfer in several bacterial species. Together, our findings show that ciprofloxacin treatment of S. maltophilia leads to the secretion of a heterogeneous pool of MVs and the induction of prophages that are potentially involved in adverse side-effects during antibiotic treatment. |
| Keywords: | Anti-Bacterial Agents, Bacterial Drug Resistance, Ciprofloxacin, Fluoroquinolones, Microbial Sensitivity Tests, Prophages, Proteomics, Secretory Vesicles, Stenotrophomonas maltophilia, Virus Activation |
| Source: | Environmental Microbiology |
| ISSN: | 1462-2912 |
| Publisher: | Wiley |
| Volume: | 19 |
| Number: | 10 |
| Page Range: | 3930-3937 |
| Date: | October 2017 |
| Official Publication: | https://doi.org/10.1111/1462-2920.13793 |
| PubMed: | View item in PubMed |
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