Item Type: | Article |
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Title: | De novo protein structure determination from near-atomic-resolution cryo-EM maps |
Creators Name: | Wang, R.Y.R. and Kudryashev, M. and Li, X. and Egelman, E.H. and Basler, M. and Cheng, Y. and Baker, D. and DiMaio, F. |
Abstract: | We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps. |
Keywords: | Cryoelectron Microscopy, Molecular Models, Monte Carlo Method, Physical Chemistry, Protein Conformation, Proteins, Time Factors |
Source: | Nature Methods |
ISSN: | 1548-7091 |
Publisher: | Nature Publishing Group |
Volume: | 12 |
Number: | 4 |
Page Range: | 335-338 |
Date: | April 2015 |
Official Publication: | https://doi.org/10.1038/nmeth.3287 |
PubMed: | View item in PubMed |
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