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De novo protein structure determination from near-atomic-resolution cryo-EM maps

Item Type:Article
Title:De novo protein structure determination from near-atomic-resolution cryo-EM maps
Creators Name:Wang, R.Y.R. and Kudryashev, M. and Li, X. and Egelman, E.H. and Basler, M. and Cheng, Y. and Baker, D. and DiMaio, F.
Abstract:We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps.
Keywords:Cryoelectron Microscopy, Molecular Models, Monte Carlo Method, Physical Chemistry, Protein Conformation, Proteins, Time Factors
Source:Nature Methods
ISSN:1548-7091
Publisher:Nature Publishing Group
Volume:12
Number:4
Page Range:335-338
Date:April 2015
Official Publication:https://doi.org/10.1038/nmeth.3287
PubMed:View item in PubMed

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