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Preexisting hypertension and pregnancy-induced hypertension reveal molecular differences in placental proteome in rodents

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Item Type:Article
Title:Preexisting hypertension and pregnancy-induced hypertension reveal molecular differences in placental proteome in rodents
Creators Name:Mary, S. and Small, H. and Herse, F. and Carrick, E. and Flynn, A. and Mullen, W. and Dechend, R. and Delles, C.
Abstract:Preexisting or new onset of hypertension affects pregnancy and is one of the leading causes of maternal and fetal morbidity and mortality. In certain cases, it also leads to long-term maternal cardiovascular complications. The placenta is a key player in the pathogenesis of complicated hypertensive pregnancies, however the pathomechanisms leading to an abnormal placenta are poorly understood. In this study, we compared the placental proteome of two pregnant hypertensive models with their corresponding normotensive controls: a preexisting hypertension pregnancy model (stroke-prone spontaneously hypertensive rats; SHRSP) versus Wistar–Kyoto and the transgenic RAS activated gestational hypertension model (transgenic for human angiotensinogen Sprague-Dawley rats; SD-PE) versus Sprague-Dawley rats, respectively. Label-free proteomics using nano LC-MS/MS was performed for identification and quantification of proteins. Between the two models, we found widespread differences in the expression of placental proteins including those related to hypertension, inflammation, and trophoblast invasion, whereas pathways such as regulation of serine endopeptidase activity, tissue injury response, coagulation, and complement activation were enriched in both models. We present for the first time the placental proteome of SHRSP and SD-PE and provide insight into the molecular make-up of models of hypertensive pregnancy. Our study informs future research into specific preeclampsia and chronic hypertension pregnancy mechanisms and translation of rodent data to the clinic.
Keywords:Liquid Chromatography, Hypertension, Pregnancy-Induced Hypertension, Placenta, Pregnancy, Protein Interaction Maps, Proteome, Proteomics, Species Specificity, Tandem Mass Spectrometry / Methods, Inbred SHR Rats, Inbred WKY Rats, Sprague-Dawley Rats, Transgenic Rats, Animals, Rats
Source:Physiological Genomics
Publisher:American Physiological Society
Page Range:259-268
Date:June 2021
Official Publication:https://doi.org/10.1152/physiolgenomics.00160.2020
PubMed:View item in PubMed

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