Helmholtz Gemeinschaft


Dynamic changes in microbiome composition following Mare's milk intake for prevention of collateral antibiotic effect

PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
[img] Other (Supplementary Material)

Item Type:Article
Title:Dynamic changes in microbiome composition following Mare's milk intake for prevention of collateral antibiotic effect
Creators Name:Kushugulova, A. and Löber, U. and Akpanova, S. and Rysbekov, K. and Kozhakhmetov, S. and Khassenbekova, Z. and Essex, M. and Nurgozhina, A. and Nurgaziyev, M. and Babenko, D. and Markó, L. and Forslund, S.K.
Abstract:INTRODUCTION: Probiotics and prebiotics are widely used for recovery of the human gut microbiome after antibiotic treatment. High antibiotic usage is especially common in children with developing microbiome. We hypothesized that dry Mare's milk, which is rich in biologically active substances without containing live bacteria, could be used as a prebiotic in promoting microbial diversity following antibiotic treatment in children. The present pilot study aims to determine the impacts of dry Mare's milk on the diversity of gut bacterial communities when administered during antibiotic treatment and throughout the subsequent recovery phase. METHODS: Six children aged 4 to 5 years and diagnosed with bilateral bronchopneumonia were prescribed cephalosporin antibiotics. During the 60 days of the study, three children consumed dry Mare's milk whereas the other three did not. Fecal samples were collected daily during antibiotic therapy and every 5 days after antibiotic therapy. Total DNA was isolated and taxonomic composition of gut microbiota was analyzed by 16S rRNA amplicon sequencing. To assess the immune status of the gut, stool samples were analyzed by bead-based multiplex assays. RESULTS: Mare's milk treatment seems to prevent the bloom of Mollicutes, while preventing the loss of Coriobacteriales. Immunological analysis of the stool reveals an effect of Mare’s milk on local immune parameters under the present conditions.
Keywords:Mare’s Milk, Microbiome, 16S rRNA Gene Sequencing, Collateral Antibiotic Effect, Intestinal Immunity, Animals, Horses
Source:Frontiers in Cellular and Infection Microbiology
Publisher:Frontiers Media SA
Page Range:622735
Date:21 April 2021
Official Publication:https://doi.org/10.3389/fcimb.2021.622735
PubMed:View item in PubMed

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library