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Systematic identification of A-to-I RNA editing in zebrafish development and adult organs

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Item Type:Article
Title:Systematic identification of A-to-I RNA editing in zebrafish development and adult organs
Creators Name:Buchumenski, I. and Holler, K. and Appelbaum, L. and Eisenberg, E. and Junker, J.P. and Levanon, E.Y.
Abstract:A-to-I RNA editing is a common post transcriptional mechanism, mediated by the Adenosine deaminase that acts on RNA (ADAR) enzymes, that increases transcript and protein diversity. The study of RNA editing is limited by the absence of editing maps for most model organisms, hindering the understanding of its impact on various physiological conditions. Here, we mapped the vertebrate developmental landscape of A-to-I RNA editing, and generated the first comprehensive atlas of editing sites in zebrafish. Tens of thousands unique editing events and 149 coding sites were identified with high-accuracy. Some of these edited sites are conserved between zebrafish and humans. Sequence analysis of RNA over seven developmental stages revealed high levels of editing activity in early stages of embryogenesis, when embryos rely on maternal mRNAs and proteins. In contrast to the other organisms studied so far, the highest levels of editing were detected in the zebrafish ovary and testes. This resource can serve as the basis for understanding of the role of editing during zebrafish development and maturity.
Keywords:Adenosine, Developmental Gene Expression Regulation, Genetic Code, Inosine, RNA Editing, Animals, Zebrafish
Source:Nucleic Acids Research
Publisher:Oxford University Press
Page Range:4325-4337
Date:7 May 2021
Official Publication:https://doi.org/10.1093/nar/gkab247
PubMed:View item in PubMed

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