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| Item Type: | Article |
|---|---|
| Title: | Activation of Smoothened in the Hedgehog pathway unexpectedly increases Gα(s)-dependent cAMP levels in Drosophila |
| Creators Name: | Praktiknjo, S.D. and Saad, F. and Maier, D. and Ip, P. and Hipfner, D.R. |
| Abstract: | Hedgehog (Hh) signaling plays a key role in the development and maintenance of animal tissues. This signaling is mediated by the atypical G protein-coupled receptor (GPCR) Smoothened (Smo). Smo activation leads to signaling through several well-characterized effectors to activate Hh target gene expression. Recent studies have implicated activation of the heterotrimeric G protein subunit Gα(i) and the subsequent decrease in cellular cAMP levels in promoting the Hh response in flies and mammals. Although Hh stimulation decreases cAMP levels in some insect cell lines, here using a bioluminescence resonance energy transfer (BRET)-based assay we found that this stimulation had no detectable effect in Drosophila S2-R+ cells. However, we observed an unexpected and significant Gα(s)-dependent increase in cAMP levels in response to strong Smo activation in Smo-transfected cells. This effect was mediated by Smo's broadly conserved core, and was specifically activated in response to phosphorylation of the Smo C-terminus by GPCR kinase 2 (Gprk2). Genetic analysis of heterotrimeric G protein function in the developing Drosophila wing revealed a positive role for cAMP in the endogenous Hh response. Specifically, we found that mutation or depletion of Gα(s) diminished low-threshold Hh responses in Drosophila, whereas depletion of Gα(i) potentiated them (in contrast to previous findings). Our analysis suggested that regulated cAMP production is important for controlling the sensitivity of cellular responses to Hh in Drosophila. |
| Keywords: | Cyclic AMP, Drosophila Proteins, Gs GTP-Binding Protein alpha Subunits, Hedgehog Proteins, Phosphorylation, Signal Transduction, Smoothened Receptor, Animals, Drosophila melanogaster |
| Source: | Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 293 |
| Number: | 35 |
| Page Range: | 13496-13508 |
| Date: | August 2018 |
| Official Publication: | https://doi.org/10.1074/jbc.RA118.001953 |
| PubMed: | View item in PubMed |
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